FastQCFastQC Report
Thu 22 Sep 2016
HL7Y3BGXY_n01_timecourse_shoot_5k2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7Y3BGXY_n01_timecourse_shoot_5k2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29669545
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAT44800.09.5339761
CGGGCAT73000.09.4958651
CGCGAAT33150.09.3993691
CTCGGAT69250.08.9484061
GCCGAAT49350.08.9387251
CGCGTAT19600.08.9311251
CCGGGAT112750.08.8184851
GGGAAAT108950.08.6440511
CGGAAAT53200.08.5550781
CGGGACT36100.08.3403351
GGAAAAT170050.08.2764021
CGGGGAT106000.08.224051
CGACAAT86050.08.2185031
CCGGAAT59450.07.95012861
GTCGGAT63750.07.85322571
CCCGTAT22450.07.7973291
CGGAAAC62850.07.6871621
GGGAGAT148550.07.494571
CGGGTAT29450.07.48940751
CGGCAAT96700.07.4581821
Produced by FastQC (version 0.11.2)