FastQCFastQC Report
Thu 22 Sep 2016
HL7Y3BGXY_n01_timecourse_shoot_5k1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7Y3BGXY_n01_timecourse_shoot_5k1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences29565391
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT100500.09.5103591
CGCGAAT31750.09.1523931
CCGGGAT104100.09.0805751
CGGCAAT93250.08.0346181
CGACAAT81350.07.87578441
CGGGCAT72600.07.8605211
CCGGAAT65800.07.7683231
GGAAAAC116850.07.7302111
CTCGGAT70300.07.71927831
CGGAAAT54200.07.68684241
GGCGAAT46650.07.6550641
GCCGAAT55900.07.6409681
CGGAGAT211700.07.6239561
CGGGAAT57450.07.49575571
GGAAAAT167850.07.46727231
GTCGAAT59550.07.466591
CCCGGAT69550.07.4501481
CCCGTAT23800.07.35518071
CGGGACT38100.07.351321
GCGGAAT41650.07.3131521
Produced by FastQC (version 0.11.2)