FastQCFastQC Report
Thu 22 Sep 2016
HL7Y3BGXY_n01_timecourse_shoot_15k1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7Y3BGXY_n01_timecourse_shoot_15k1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22609869
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT24150.010.1476711
CGGCAAT76400.09.1647821
GGAAAAT123350.08.5998121
CGGGCAT57000.08.4759751
CCCGTAT17100.08.3940821
GGGATAT59950.08.3508831
CGACAAT67050.08.3020211
GGGAAAT82900.08.2772671
CGGGGAT77850.08.1396441
GCGGAAT31150.08.0920741
CGGAAAT37800.08.05773451
GGGAGAT118400.07.80616431
CGGGTAT23400.07.7798811
GGAAAAC91350.07.70323231
CCCGAAT28200.07.6971161
CGGACAT64800.07.6718271
CGGGAAT39800.07.6528231
CCGGGAT81050.07.6454971
CGGATAT34850.07.6347751
GGGGAAT62800.07.5816681
Produced by FastQC (version 0.11.2)