FastQCFastQC Report
Thu 22 Sep 2016
HL7Y3BGXY_n01_timecourse_shoot_10n3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7Y3BGXY_n01_timecourse_shoot_10n3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7733667
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT8250.011.4580291
GCCGAAT14550.011.0686571
CCCGAAT9100.010.38777351
CCGGGAT26150.010.0412931
GGGGAAT19950.010.003041
CGAATGT9400.09.6780343
CGGGCAT19300.09.2515171
CGGGTAT7604.7475623E-109.2133271
CGGAAAT13700.08.9443251
GCGGAAT9901.8189894E-128.8410711
CGGATAT11950.08.7892831
CGGGGAT25800.08.549111
CCCGGAT16000.08.5338441
GGGAAAT27950.08.5177951
GGCGAAT12350.08.504611
CGGCTAT10851.8189894E-128.38964751
GGGAGAT39300.08.3740471
CGGAGAT55150.08.3162171
CCGCAAT12750.08.2377991
GTCGAAT17500.08.2024941
Produced by FastQC (version 0.11.2)