FastQCFastQC Report
Thu 22 Sep 2016
HL7Y3BGXY_n01_timecourse_shoot_10n2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7Y3BGXY_n01_timecourse_shoot_10n2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6667433
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT5855.4242264E-910.1749061
CTCGAAT13800.010.1488831
GCCGAAT11200.09.6912771
GGCGAAT11300.09.6055141
CGGGAAT12400.09.3181481
CGGGATT10600.09.2488861
CGGGGAT22950.09.1538931
CGACAAT19350.09.0474531
GGGGAAT18800.08.9396531
CCCGTAT5552.8516097E-68.8322711
GGGAGAT34200.08.8046011
GGGAAAT23700.08.7164761
CGGCTAT8453.8016879E-108.7016151
CGGAAAC13850.08.3426021
CTCGGAT15650.08.2779871
CGCGAAT7201.5840851E-78.2671111
CCGGGAT25000.08.263221
GGGATAT17700.08.1105051
GCGGAAT8402.7486749E-87.91975261
GGAAAAT38200.07.8826531
Produced by FastQC (version 0.11.2)