FastQCFastQC Report
Thu 22 Sep 2016
HL7Y3BGXY_n01_timecourse_shoot_10n1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7Y3BGXY_n01_timecourse_shoot_10n1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18310853
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT46000.010.7323271
CGCGAAT21250.09.8860991
CGGGGAT64550.09.872061
CGGGACT22350.09.5561931
CGACAAT48950.08.7980221
CGGGTAT18350.08.7771681
GGGAAAT65400.08.5659371
GGAAAAT97550.08.5424471
CCGGGAT69250.08.4941931
GGGGAAT53350.08.4661881
GGCGAAT29350.08.3506941
CGGAGAT133100.08.1022431
CGGCTAT26800.08.1000841
GGAAAAC70100.07.9916161
GGGACAT50450.07.91181851
CGGGAAT32350.07.90098431
GGATCTA39000.07.8952734
GCGGAAT23450.07.76413541
GCGAATC39150.07.68654542
CGGCAAT56650.07.54036761
Produced by FastQC (version 0.11.2)