FastQCFastQC Report
Thu 22 Sep 2016
HL7Y3BGXY_n01_timecourse_shoot_10k3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7Y3BGXY_n01_timecourse_shoot_10k3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences5742288
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT20650.010.1707611
CGGAAAT10600.09.9068971
CGCGAAT6101.0815711E-89.7553171
CGTAACG2950.00453389869.49610242
CTCGAAT11400.08.904621
CGGCAAT17900.08.7999821
CCGGGAT21500.08.7917951
CGGCTAT8001.2551027E-98.7510921
GGGATAT15750.08.6677481
CGGAAAG11750.08.6393771
CCCGACT6908.015195E-88.6242651
CGGACAT14700.08.5724981
GGAAGAT49850.08.5667671
CTCGGTT10650.08.5456681
GGGAGAT29150.08.5259351
CGACAAT16400.08.324211
CTCGGAT13950.08.2806031
CGGGCAT13750.08.1464711
CGGGTAT6059.136349E-68.1001841
GGAAAAT30750.08.0823091
Produced by FastQC (version 0.11.2)