FastQCFastQC Report
Thu 22 Sep 2016
HL7Y3BGXY_n01_timecourse_shoot_10k2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7Y3BGXY_n01_timecourse_shoot_10k2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15071852
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAT26950.010.0027731
CGGGCAT35350.09.50758651
CGCGAAT16800.09.37761
CGGGACT20500.08.8805111
CCGGGAT54100.08.67153551
GGGGTAT20250.08.6443721
GGAAAAT85250.08.5419581
CGCGTAT11150.08.4776871
CGGCTAT21350.08.3629751
CGACAAT41100.08.2626311
CCCGAAT17200.07.9382471
CTCGTAT20800.07.91084671
CGGAAAC31750.07.82894231
GGAAGAT130250.07.76798871
CGACGAT113250.07.6665851
CGGGGAT52600.07.65421341
CTCGGAT37650.07.6249561
CTCGAAT29850.07.6235541
CGGAAAT26500.07.53038931
GGGATAT41400.07.52624131
Produced by FastQC (version 0.11.2)