FastQCFastQC Report
Thu 22 Sep 2016
HL7Y3BGXY_n01_timecourse_shoot_10k1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7Y3BGXY_n01_timecourse_shoot_10k1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences23102828
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGTAT23550.010.8527221
CCCGACT29150.09.2482371
GGGAAAT81550.09.144551
CGGGGAT81200.09.0977331
CCGGGAT84650.09.0578241
CGGGCAT59150.08.7601941
CGCGAAT25300.08.7181991
CGGGAAT38750.08.6737371
GGAAAAT124900.08.4935051
CGGAAAT39800.08.4449071
GCCGAAT41500.08.4364291
GGGAGAT114900.08.0443441
CGGAGAT167200.07.9570861
GTCGAAT44100.07.85965251
GGGGAAT64300.07.84076171
CGACAAT65800.07.7684381
GGAAGAT205200.07.7120141
CGGAAAC48100.07.6427731
CTGGAAT112400.07.63144061
CGGCTAT31450.07.56998441
Produced by FastQC (version 0.11.2)