FastQCFastQC Report
Thu 22 Sep 2016
HL7Y3BGXY_n01_timecourse_shoot_02.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7Y3BGXY_n01_timecourse_shoot_02.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28816442
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT32700.09.1003741
CCGGGAT98100.08.707811
CTCGGAT67050.08.6675721
CTCGAAT54000.08.5579211
CTCGTAT42000.08.5023511
CGGGGAT100400.08.3339781
CTGGAAT160400.08.29406451
CTGGGAT121500.08.0968891
CGGGAAT50450.07.9804031
GGGAAAT109000.07.9012671
CGGCAAT86350.07.86552141
GGAAGAT262300.07.8481481
GGGAGAT141500.07.66996531
CGGGCAT67300.07.6469881
GTCGGAT64500.07.5991
CGGAAAC59000.07.5359821
GGAAAAT158350.07.450751
CGACAAT81100.07.4249881
GGGGAAT88400.07.32668641
GGGAAAC104900.07.2422311