FastQCFastQC Report
Thu 22 Sep 2016
HL7Y3BGXY_n01_timecourse_shoot_01.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7Y3BGXY_n01_timecourse_shoot_01.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences28068558
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT65900.010.0412131
CGCGAAT30300.09.8216871
CTCGAAT57300.08.9819881
CGGGACT34650.08.7907481
GGGGAAT81750.08.6083141
CGGGTAT27850.08.5485711
GGAAAAT160800.08.448031
CGGGGAT90050.08.4369561
GGGAAAT108050.08.3275671
GGAAAAC118450.08.1284441
GGAAGAT247050.08.0637521
GCCGAAT50700.08.0105041
CTGGGAT121800.07.76115371
CTGGCAT128650.07.7561261
GGGAGAT142950.07.71500441
CGGGAAT47700.07.6335181
CGGAAAT52300.07.49766641
GGCGAAT44000.07.47971341
CTCGGAT73600.07.46846961
CCCGACT55800.07.4665941