FastQCFastQC Report
Wed 5 Oct 2016
HL7VKBGXY_n01_timecourse_shoot_90n3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7VKBGXY_n01_timecourse_shoot_90n3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15235238
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTCCGAT38500.010.0872972
GGGAAAC52300.010.0399871
GCCGAAT28050.09.983911
CGGGGAT57450.09.6883681
CCGGGAT55200.09.5125231
GGAAAAT84100.09.4903541
GGCGAAT25100.09.483721
GGGGAAT41850.09.4520621
GCGGAAT20450.09.414841
CTTCCGA43300.09.3780741
GGGAAAT61000.09.0671491
CGGGTAT14350.09.0259591
GTCGAAT31650.08.6270911
CGCGAAT15650.08.499881
GGGAGAT76200.08.4069731
CGGGCAT36850.08.3596621
CTCGGAT33000.08.2741661
TCCCTAC29250.08.0136729
GCCAGAT74400.07.9987021
CGGGAAT26850.07.9529651
Produced by FastQC (version 0.11.2)