FastQCFastQC Report
Wed 5 Oct 2016
HL7VKBGXY_n01_timecourse_shoot_90n2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7VKBGXY_n01_timecourse_shoot_90n2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14084270
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAGGT20800.010.7649211
ACCTAGG20500.010.75179310
ATACCTA25500.010.5643988
GGAAAAT77550.010.427171
GGAAAAC55500.010.1547491
GGGGAAT39700.09.9637831
GCCGAAT25550.09.8645641
GGGAGAT68050.09.6194391
CGGGCAT30250.09.60481551
CGTTAGC16850.09.55147923
GGGAAAC53850.09.4258081
GGGAAAT57450.09.3226151
GCCGGAT49650.09.0245811
CTAAACG19150.08.95441245
TTCCGAT38400.08.9294462
CCGGGAT43550.08.7614231
GTCGAAT30250.08.679051
CTTCCGA39700.08.6411571
GCCAGAT69200.08.5996181
TACCTAG28100.08.5908669
Produced by FastQC (version 0.11.2)