FastQCFastQC Report
Wed 5 Oct 2016
HL7VKBGXY_n01_timecourse_shoot_90n1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7VKBGXY_n01_timecourse_shoot_90n1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17654738
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGAAT34300.011.225961
CGGGCAT36700.010.5872171
GGGAAAT71750.010.3916041
GGAAAAT99500.010.37824251
GGGAAAC67750.010.0234521
GGGGAAT49850.09.9009951
GCCGAAT34300.09.7972011
GCGGAAT22200.09.4606971
CCGGGAT51450.09.3889841
TCCCTAC36350.09.3360549
GGCGAAT31300.09.2823651
GCCAAAT78150.09.0926811
CGCGAAT19150.08.9567861
TTCCGAT46900.08.8776842
GGGATAT51500.08.7001681
CTTCCGA49500.08.4859591
CGGGGAT54450.08.4859591
CGGAGAT109650.08.459841
GCCAGAT89950.08.4057691
GCCGGAT58300.08.2257771
Produced by FastQC (version 0.11.2)