FastQCFastQC Report
Wed 5 Oct 2016
HL7VKBGXY_n01_timecourse_shoot_90k3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7VKBGXY_n01_timecourse_shoot_90k3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20059484
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGGAT59450.010.1272851
GGGAAAT79350.010.0137181
CGGGCAT43100.09.9895121
CGCGAAT23500.09.6819311
GCCGGAT65350.09.6414721
GCGGAAT27200.09.26573851
GGGGAAT56550.09.2229561
GGAAAAT114950.09.1354241
CGCGTAT15050.09.0707791
CGGGTAT22800.09.0580271
TCCCTAC43300.09.0493429
CCGGGAT62500.09.0170051
GGGGTAT29900.08.7802421
GGGAGAT103100.08.7255121
GGCGAAT36650.08.5002651
CGGGACT25400.08.2686391
CGGCTAT29750.08.2362121
GGGAAAC74500.08.2223931
CCCGGAT39100.08.14668751
CGGAAAT36950.08.0523191
Produced by FastQC (version 0.11.2)