FastQCFastQC Report
Wed 5 Oct 2016
HL7VKBGXY_n01_timecourse_shoot_90k2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7VKBGXY_n01_timecourse_shoot_90k2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17968480
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA52250.010.3833411
GCGGAAT23000.010.3483911
TTCCGAT53200.09.930552
CGGGTAT19800.09.8995291
GGGAAAT67650.09.5201041
CGGGCAT38450.09.3763251
GGCGAAT32150.09.3628741
GCCGGAT59950.09.2832411
CGGGGAT53600.09.2728931
CGGGAAT34050.09.2516051
CCGGAAT35750.09.0074841
GCCGAAT32800.08.9639031
GGGAAAC67750.08.8860931
GCCGATT37300.08.7270171
GGAAAAT102400.08.6479321
CCGGGAT51950.08.556771
TCCCTAC36200.08.5047979
CGGAAAC40500.08.3831731
GGGAGAT89150.08.3235051
GGGACAT46500.08.2800211
Produced by FastQC (version 0.11.2)