FastQCFastQC Report
Wed 5 Oct 2016
HL7VKBGXY_n01_timecourse_shoot_90k1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7VKBGXY_n01_timecourse_shoot_90k1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14788383
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGGCAT32300.012.6792821
TTCCGAT41550.010.8626972
CTTCCGA42900.010.8518981
GCGGAAT18850.010.7703051
GGGAAAT61500.010.1880071
GCCGAAT29350.09.8987721
CGGGGAT47850.09.7292881
TCCCTAC33300.09.5604099
CGGAAAT26100.09.387911
GCGGGAT35100.09.2744281
CCGGGAT45750.09.10473251
TTCCCTA36700.08.9606838
GGAAAAT80100.08.95844651
CGGCAAT45400.08.8665231
GGGAAAC53150.08.4956731
GCCAAAT64700.08.4397921
GCCGATT31150.08.4278071
CGCGTAT10851.8189894E-128.3879331
CCGGAAT29950.08.2979891
GGGAGAT73800.08.2054251
Produced by FastQC (version 0.11.2)