FastQCFastQC Report
Wed 5 Oct 2016
HL7VKBGXY_n01_timecourse_shoot_60n3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7VKBGXY_n01_timecourse_shoot_60n3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18799218
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGAAT23000.010.8052541
TTCCGAT52150.010.3987982
CCGGGAT65100.010.3234461
TCCCTAC41000.010.2396129
CGGGGAT65850.010.2058671
CTTCCGA54100.010.15796851
GGAAAAT101050.09.8721791
GGGAAAT64100.09.6653971
GGGAGAT98750.09.2159621
CGGGAAT32200.09.1312011
TTCCCTA43550.09.0777368
GGGAAAC60950.09.0163431
CGGGCAT44600.08.8684571
CGGCTAT27700.08.7191431
GGGACAT51500.08.6317921
GGAAAAC70800.08.6024161
CGGAAAT32950.08.4984371
GCCAGAT88800.08.474811
CCCTACA48700.08.40512510
GGCGAAT33450.08.3714051
Produced by FastQC (version 0.11.2)