FastQCFastQC Report
Wed 5 Oct 2016
HL7VKBGXY_n01_timecourse_shoot_60n2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7VKBGXY_n01_timecourse_shoot_60n2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16766629
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT237560.14168620299286158No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGCGAAT19200.011.1205461
CTTCCGA47400.010.7813221
TTCCGAT49800.010.3274352
CCGGGAT48700.09.9904111
GCCGAAT30000.09.8006641
GGGGTAT26900.09.7590271
GGAAAAT91000.09.69296551
GGGAAAT64650.09.6371581
GGCGAAT26500.09.3779951
GGGAAAC65100.09.1941721
GCGGGAT40600.09.0523391
GCCGGAT52850.08.9410041
CGGGGAT51450.08.91216851
GGGGAAT47600.08.677061
CGGGATA22300.08.6290362
CGCGTAT13800.08.6237741
CGGGAAT34300.08.3679141
GCGGAAT21500.08.3028891
GCCAGAT74850.08.2303511
GGGACAT42150.08.2211981
Produced by FastQC (version 0.11.2)