FastQCFastQC Report
Wed 5 Oct 2016
HL7VKBGXY_n01_timecourse_shoot_60n1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7VKBGXY_n01_timecourse_shoot_60n1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16369404
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA49400.010.8412391
TTCCGAT48600.010.6546582
CGGGTAT17750.010.0574131
TCCCTAC35350.09.8971179
GCCGAAT29550.09.7133891
GGGAAAT62600.09.6735571
GGGAAAC63300.09.4006871
CGGGGAT54000.09.0102211
GGCGAAT26850.08.9953741
CGGGCAT34800.08.95210551
GGAAAAT94250.08.9134191
GCGGAAT22650.08.80888751
GGGGTAT23350.08.6947191
CGCGAAT18250.08.6310581
GGGGAAT48500.08.5885451
GCGGTAT16850.08.517231
TTCCCTA39250.08.4680238
GCGGGAT38500.08.3645291
GGGACAT45750.08.3396881
CCCGTAT14700.08.3342231
Produced by FastQC (version 0.11.2)