FastQCFastQC Report
Wed 5 Oct 2016
HL7VKBGXY_n01_timecourse_shoot_60k3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7VKBGXY_n01_timecourse_shoot_60k3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19357882
Sequences flagged as poor quality0
Sequence length76
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAAT103200.010.9207921
GGCGAAT30650.09.9349731
CCGGGAT79550.09.7676641
CGCGAAT22050.09.3652931
CGGGGAT75050.09.2806911
GCCGAAT35900.09.0670581
CGGGCAT47300.09.0276891
GGGGAAT53400.08.6518821
TTCCGAT48700.08.6209952
CGCAAAT34050.08.5317651
GAAAAAT121450.08.5016351
CGGGAAT33700.08.4126541
TTCCCTA38950.08.3533028
GGGAAAT70550.08.3347031
TCCCTAC38300.08.3123799
GGGAGAT103900.08.28700351
CCGGAAT38450.08.1926441
CTTCCGA53900.08.1820041
CTCGGAT42600.08.1339881
GCCAGAT90150.08.1144391
Produced by FastQC (version 0.11.2)