FastQCFastQC Report
Wed 5 Oct 2016
HL7VKBGXY_n01_timecourse_shoot_60k2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7VKBGXY_n01_timecourse_shoot_60k2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22256946
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAAAT81250.010.037981
CGGGCAT54800.09.83680251
CGGAAAT38800.09.6530781
CGGGGAT79450.09.6045361
CCCGTAT17200.09.56496051
CGCGAAT22800.09.5185461
GGAAAAT126400.09.1109291
CCGGGAT81300.08.7401581
GGGGAAT64000.08.6415351
GCCGAAT39600.08.2205631
GGCGAAT38800.08.2096271
GGCAAAT97300.08.1663271
GGGAGAT108250.08.1163281
CGGCTAT31400.08.0263231
CGGGAAT38050.08.0034711
CGGCAAT71300.08.0022451
GGAAAAC88100.07.9860851
GGGAAAC75450.07.9332411
GTCGAAT41900.07.8528561
CGCAAAT41550.07.834761
Produced by FastQC (version 0.11.2)