FastQCFastQC Report
Wed 5 Oct 2016
HL7VKBGXY_n01_timecourse_shoot_60k1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7VKBGXY_n01_timecourse_shoot_60k1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18881508
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT381590.20209720537152012No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGAAT34500.09.9426371
TTCCGAT51250.09.7623642
CGGGCAT41150.09.4416471
GGCGAAT30050.09.4348541
CCGGGAT61850.09.3376771
CTTCCGA55150.09.3296731
GCGGAAT26000.09.1544071
GCGGGAT47900.08.4034371
GGAAAAT110950.08.3916811
GGGGAAT50500.08.317341
GCGAAAT39450.08.2514551
CGGGGAT62100.08.1164391
GGGAAAT71750.08.0004891
GCGGATT35200.07.9550321
CCGGAAT37300.07.6948411
GGGAGAT93000.07.6026151
GGGAAAC64400.07.50045871
CGGCAAT60900.07.47172161
CTCGGAT43850.07.4234871
CGGATAT26900.07.41681051
Produced by FastQC (version 0.11.2)