FastQCFastQC Report
Wed 5 Oct 2016
HL7VKBGXY_n01_timecourse_shoot_45n3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7VKBGXY_n01_timecourse_shoot_45n3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19307458
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2483671.2863785590003614No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA885020.45838245511138753No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA58400.015.4028161
TTCCGAT56700.015.2416012
TATGCCG33900.010.32299946
TCGTATG32100.010.1386543
GCGGAAT27300.010.0002721
CCGATCT86100.09.9148334
GCGGGAT43850.09.8976471
CGTATGC35800.09.77523144
TCCGATC87350.09.6128373
CTCGTAT33800.09.42118542
CCGGGAT63750.09.2786841
ACTTAGG36100.09.20843832
GGGAAAT71150.08.8547731
GCGAAAT38900.08.7277431
GCGGTAT18550.08.4907961
CGGGCAT45100.08.3039951
CTTAGGC39650.08.29583133
CGGGAAT31950.08.2161861
CCGGAAT38000.07.9212681
GGAAAAT110700.07.90446041
Produced by FastQC (version 0.11.2)