FastQCFastQC Report
Wed 5 Oct 2016
HL7VKBGXY_n01_timecourse_shoot_45n2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7VKBGXY_n01_timecourse_shoot_45n2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17858965
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2272081.2722349811425242No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA663170.37133730874101606No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGAAT26650.010.9012941
GCCGAAT29950.09.9338881
TTCCGAT50850.09.7700672
CTTCCGA51400.09.6018111
GGGAAAT67500.09.4895251
CGGGCAT41600.09.0871481
CCGGAAT34050.09.0461281
CGGGGAT59500.08.8829481
TCGTATG27100.08.78110643
GGAAAAT102350.08.6522661
CTCGTAT28950.08.34047242
TATGCCG30700.08.20745646
CGTATGC29500.08.06684644
TCCCTAC35500.07.9823829
GCGAAAT35550.07.97521831
TATCTCG32200.07.93262639
CTACACG33350.07.86758112
GCGGTAT17800.07.86569641
GCGGGAT48600.07.8503211
CGGACAT47950.07.8107431
Produced by FastQC (version 0.11.2)