FastQCFastQC Report
Wed 5 Oct 2016
HL7VKBGXY_n01_timecourse_shoot_45n1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7VKBGXY_n01_timecourse_shoot_45n1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15727745
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT1783661.1340850198168904No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA573050.36435611080927366No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCGGAAT21350.010.8203181
CTTCCGA45100.010.7877851
TTCCGAT44000.010.7346012
CCGGGAT52350.09.3606361
CGGGCAT35950.09.249511
GGGAAAT62750.09.0363971
TCGTATG24150.08.98424443
GCCGAAT28450.08.9812051
CGGGGAT50750.08.6901751
TATGCCG25800.08.54544646
CGGAAAT27500.08.5277821
CGGGACT17250.08.5222491
GCGAAAT35000.08.5005081
GGAAAAT92350.08.4520481
GGCGAAT24950.08.4173361
ACCTAGG20850.08.2219110
GCGGGAT41350.08.1262421
TCCCTAC31300.07.93585979
GGGAGAT74850.07.85618161
CGGGTAT17950.07.79990961
Produced by FastQC (version 0.11.2)