FastQCFastQC Report
Wed 5 Oct 2016
HL7VKBGXY_n01_timecourse_shoot_120n3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7VKBGXY_n01_timecourse_shoot_120n3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20127469
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGTAA27450.013.12971236
CGTAATC27500.012.97850238
TCGTATG30850.012.47838245
ACGTAAT30150.011.489637
CGTATGC35850.010.64037646
CTCGTAT32500.010.4449944
ACCGTAC36800.010.36466332
CCGGGAT64950.010.2941241
TATGCCG34700.010.28721748
GCGGAAT24500.010.0015451
CGGGCAT47100.09.5874681
GGAAAAT109400.09.4712991
CGGGGAT67350.09.4595171
CGCGTAT16350.09.4204161
GGGAAAT83400.09.1500951
GGGAGAT99750.09.1242161
GCCAGAT99950.09.1059591
CGGGAAT33250.09.054031
GGGGAAT55050.09.0295511
TCCCTAC42050.08.8195479
Produced by FastQC (version 0.11.2)