FastQCFastQC Report
Wed 5 Oct 2016
HL7VKBGXY_n01_timecourse_shoot_120n2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7VKBGXY_n01_timecourse_shoot_120n2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14674346
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GCCGAAT25950.010.3858431
CGGGAAT25100.010.1797611
GGGAAAT58100.010.1209571
CTTCCGA40300.09.7275041
GGGAAAC51300.09.6885631
TTCCGAT40600.09.4792612
CTCGGAT30000.09.21711
GGGGTAT20900.09.21095851
GGAAAAT82700.09.1419041
GCGGAAT19750.09.0383991
TTCCCTA32700.08.9869288
CGGGTAT14950.08.8967391
GCCAGAT72000.08.8962521
TCCCTAC30650.08.5607249
GGCGAAT25100.08.5063761
GGGAGAT69900.08.4624861
GGGGAAT41050.08.3560561
CCGGGAT44900.08.3411481
CGGGCAT32200.08.2612581
GCCAAAT61550.08.2457161
Produced by FastQC (version 0.11.2)