FastQCFastQC Report
Wed 5 Oct 2016
HL7VKBGXY_n01_timecourse_shoot_120n1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7VKBGXY_n01_timecourse_shoot_120n1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12759653
Sequences flagged as poor quality0
Sequence length76
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGAAAAT71350.010.4495251
CGGGCAT28850.010.3129771
GCGGAAT17500.010.0009881
GCCGAAT23000.09.4357151
CTTCCGA35350.09.406871
GGCGAAT21600.09.3990761
CGGCAAT38850.09.2801971
CGGGGAT44750.09.2299621
TTCCGAT32650.09.1085492
CCGGGAT44700.09.0836721
TCCCTAC23150.09.0677279
GCGAAAT27850.08.79799651
GGGAAAT51150.08.75941851
CGGAAAT21150.08.440551
CGGGTAT12750.08.2361081
CGGGAAT21800.08.1888821
CGCGGAT37050.08.124961
GGGAGAT62500.07.6167531
GCGGAAA23900.07.61581471
GCCGGAT48500.07.5058961
Produced by FastQC (version 0.11.2)