FastQCFastQC Report
Wed 5 Oct 2016
HL7VKBGXY_n01_timecourse_shoot_120k3.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7VKBGXY_n01_timecourse_shoot_120k3.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12021263
Sequences flagged as poor quality0
Sequence length76
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTTCCGA32050.011.1399761
CGGGCAT24950.010.8026981
CGCGAAT14700.010.4772531
TTCCGAT34050.010.2751692
CGGGGAT37600.09.7749271
GGGAAAT49050.09.7053681
GGGGAAT34500.09.23282051
CGTATCT11000.08.9056223
GGAAAAT70400.08.900051
GGGGTAT19050.08.8197921
TCCCTAC24950.08.693799
GGCGAAT22300.08.63316251
CGGGTAT13150.08.5179741
GCCGGAT40400.08.490961
CGGAAAT21700.08.3879481
CGGGACT14700.08.3341791
CCGGGAT33250.08.2113591
GTCGAAT23450.08.2097881
GGAAAAC52200.08.1809071
CGGGAAT23350.08.0950391
Produced by FastQC (version 0.11.2)