FastQCFastQC Report
Wed 5 Oct 2016
HL7VKBGXY_n01_timecourse_shoot_120k2.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7VKBGXY_n01_timecourse_shoot_120k2.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12896125
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAAAT50400.010.1400431
CGCGAAT14600.010.0696391
CCGGGAT41150.09.6122731
CCCGAAT14650.09.5574021
GGAAAAT76900.09.376881
GGGAAAC48200.09.1504191
CGGGAAT23300.09.0139021
GGGATAT32700.08.9918491
CGGGGAT41300.08.6450511
CCCGTAT12150.08.6429591
TTCCGAT32650.08.5731552
CGGGTAT12250.08.5724041
CTTCCGA33700.08.517291
CTCGGAT28800.08.3863711
GCCAAAT60100.08.2704921
CGGGACT16150.08.2362321
CGGGCAT29850.08.09137251
CCCGGAT27350.08.0630751
GCCGAAT22450.07.9519071
GTCGAAT24250.07.9390481
Produced by FastQC (version 0.11.2)