FastQCFastQC Report
Wed 5 Oct 2016
HL7VKBGXY_n01_timecourse_shoot_120k1.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7VKBGXY_n01_timecourse_shoot_120k1.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12784520
Sequences flagged as poor quality0
Sequence length76
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGAAAT50250.09.8216271
GCCGGAT43500.09.6558911
CCGGAAT22350.09.5532851
GCCGAAT20650.09.4922311
CGGGAAT22600.09.4476061
GGCGAAT21400.09.3231241
GCGGAAT15800.09.0829541
CGGGTAT13700.08.9422721
CCGGGAT39000.08.8852771
CTTCCGA36650.08.8819711
GCCAAAT55050.08.83807851
CCCGTAT11100.08.8294861
GGGAGAT64450.08.7438621
GGGGTAT18900.08.7043511
CGCAAAT23950.08.4766231
CGGGCAT26600.08.42167951
CGGGGAT42150.08.3873391
TTCCGAT35500.08.3770382
CGGCAAT38850.08.2889051
TCCCTAC27300.07.9453929
Produced by FastQC (version 0.11.2)