FastQCFastQC Report
Fri 14 Oct 2016
HL7T3BGXY_n01_sv153.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7T3BGXY_n01_sv153.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences70195671
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGCTACATCTCGTATGCC7256881.033807341196297TruSeq Adapter, Index 11 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGGCTAC806750.042.8379633
ACGGCTA815500.042.69515632
GATCGGA831750.042.618821
CTCGTAT812750.042.354542
TCGTATG818000.042.35206643
TCTCGTA811250.042.33429741
CGTATGC818350.042.3338944
CGTCTGA833500.042.32289516
ATCGGAA849750.042.209442
TCACGGC827200.042.12107530
GTCACGG830100.042.0497229
ACGTCTG842650.041.9037715
CACGGCT834300.041.76267631
ACACGTC851400.041.5951213
ATCTCGT825450.041.56603640
TCGGAAG862100.041.5280653
AGTCACG841900.041.51377528
CATCTCG827150.041.49429339
CGGAAGA864550.041.3456084
GTATGCC854900.040.6002945