FastQCFastQC Report
Fri 14 Oct 2016
HL7T3BGXY_n01_sv152.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7T3BGXY_n01_sv152.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences45923553
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTAGCTTATCTCGTATGCC3963660.8630995951031925TruSeq Adapter, Index 10 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTATGC455100.042.31354544
TCGTATG446150.042.219443
CTCGTAT450550.042.17671242
TCTCGTA454250.042.06252341
GATCGGA496400.042.0048451
CGTCTGA504050.041.65450716
ATCGGAA508750.041.636092
TATCTCG468600.041.361339
ACGTCTG509100.041.2592215
ACACGTC511000.041.18991513
ATCTCGT470250.040.98641640
TCGGAAG520400.040.7145083
CGGAAGA526650.040.1935274
GTATGCC486850.039.89137645
CACGTCT534050.039.38654314
GCACACG535350.039.36355211
CACACGT537100.039.2557312
GCTTATC520650.039.13677236
ACTAGCT548550.037.98937632
TAGCTTA548700.037.74235534
Produced by FastQC (version 0.11.2)