FastQCFastQC Report
Fri 14 Oct 2016
HL7T3BGXY_n01_sv151.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7T3BGXY_n01_sv151.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences71673571
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGATCAGATCTCGTATGCC11929961.6644852256628877TruSeq Adapter, Index 9 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA1337900.043.4150621
ATCGGAA1359450.043.226732
TCGGAAG1375400.042.5525633
CGGAAGA1377800.042.387274
CTCGTAT1334700.041.89634742
CGTATGC1358500.041.8957744
TCGTATG1358150.041.88174443
CGATCAG1358350.041.8789333
CGTCTGA1373900.041.86667316
GTATGCC1361300.041.86410545
TCTCGTA1333050.041.85337441
ACACGTC1383800.041.80517213
GTCACGA1363300.041.77860629
ACGATCA1368400.041.6776532
GATCTCG1333000.041.66740839
ACGTCTG1385900.041.59542515
CACGATC1374650.041.46501531
TCACGAT1377300.041.38841630
ATCTCGT1348600.041.2386940
AGTCACG1387750.041.05709528
Produced by FastQC (version 0.11.2)