FastQCFastQC Report
Fri 14 Oct 2016
HL7T3BGXY_n01_ba51.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7T3BGXY_n01_ba51.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34287101
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT17850.016.7641242
TCGTATG18300.015.98295543
TCTCGTA19450.015.50126341
CGTATGC20150.015.18544144
AATCTCG25250.012.208117539
ATCTCGT27500.011.12745240
ACACGTC42500.08.52320713
CGTCTGA41700.08.41687416
ACGTCTG46800.07.98043215
GATCGGA44800.07.836891
ATCGGAA46950.07.5244452
CACGTCT55950.06.675340714
GTATGCC52300.06.49589245
TCGGAAG59550.06.34826473
CGGAAGA61350.06.0519724
ATCGCGT4750.00619360535.684302340
GCACACG69050.05.441574611
ACGACGC6854.3705944E-45.255275713
CACACGT72550.05.179035712
CACGACG7005.9340824E-45.142707312
Produced by FastQC (version 0.11.2)