FastQCFastQC Report
Fri 14 Oct 2016
HL7T3BGXY_n01_ba50.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7T3BGXY_n01_ba50.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences30042940
Sequences flagged as poor quality0
Sequence length51
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG23450.026.86439543
CTCGTAT23200.026.76610442
TCTCGTA24300.025.55553241
CGTATGC25500.024.7929444
ATCTCGT32950.019.46180540
CATCTCG40200.015.89593139
CGTCTGA41950.015.39259516
ACACGTC42800.015.08712713
GATCGGA43100.014.88161851
ATCGGAA44950.014.5670932
ACGTCTG47300.013.79436315
GTATGCC52550.012.63024945
CACGTCT54050.012.23818914
TCGGAAG59900.011.0821393
CACACGT65250.010.55145212
CGGAAGA66450.010.294494
GCACACG71000.09.41183911
CTTAGGC78650.08.61086833
TAGGCAT81250.08.28025435
TTAGGCA84800.08.03964534
Produced by FastQC (version 0.11.2)