FastQCFastQC Report
Fri 14 Oct 2016
HL7T3BGXY_n01_ba49.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7T3BGXY_n01_ba49.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences37113165
Sequences flagged as poor quality0
Sequence length51
%GC44

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATCACGATCTCGTATGCC6884501.8550021266038614TruSeq Adapter, Index 1 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GATCGGA779100.043.3414231
ATCGGAA793500.043.0601772
TCGTATG774300.042.90892843
TCTCGTA756900.042.86240441
CTCGTAT761050.042.85499242
CGATCTC753300.042.75454738
CGTATGC778400.042.7319444
CGTCTGA787750.042.69571316
CACGATC755900.042.59867536
ACGATCT755650.042.5977637
GATCTCG758350.042.55285339
ACACGTC793600.042.5033513
TCACGAT771750.042.4991435
ATCACGA778600.042.4223834
ATCTCGT761350.042.39688540
TCGGAAG805150.042.3614433
ACGTCTG795200.042.3298915
CATCACG786950.042.20579533
CGGAAGA808450.042.1189424
GTATGCC791550.042.07604245