FastQCFastQC Report
Fri 14 Oct 2016
HL7T3BGXY_n01_ba48.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7T3BGXY_n01_ba48.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences41214096
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCTTGTAATCTCGTATGCC5076751.2317994309519733TruSeq Adapter, Index 12 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CTCGTAT547850.043.0144142
TCGTATG558900.043.01321443
TCTCGTA549100.042.91867441
CGTATGC561800.042.8871944
GATCGGA568250.042.7386931
AATCTCG554300.042.5049539
CGTCTGA576600.042.44165416
ATCGGAA580800.042.4110452
ATCTCGT558800.042.19069340
ACACGTC583950.041.99662813
ACGTCTG584300.041.90175615
TCGGAAG591400.041.591223
CGGAAGA598550.041.0648164
CACGTCT598300.041.0116714
GTATGCC591300.040.86549845
CACACGT611300.040.1623212
GCACACG611400.040.11944211
GTAATCT605350.039.02099637
TGTAATC631400.037.49675436
CCTTGTA651000.037.3202833
Produced by FastQC (version 0.11.2)