FastQCFastQC Report
Fri 14 Oct 2016
HL7T3BGXY_n01_ba47.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7T3BGXY_n01_ba47.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences40797
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATCTCGTATGCC11032.703630168884967TruSeq Adapter, Index 5 (100% over 51bp)
GATCGGAAGAGCACACGTCTGAACTCCAGTCACACAGTGATGTCGTATGCC830.20344633183812535TruSeq Adapter, Index 5 (98% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACACGTC1550.045.013
ACGTCTG1400.045.015
CGTCTGA1400.045.016
CACACGT1600.043.5937512
TCGGAAG1600.043.593753
GAGCACA1600.043.593759
ATCGGAA1600.043.593752
GCACACG1600.043.5937511
GATCGGA1450.043.448281
CGGAAGA1650.042.2727284
CACGTCT1600.042.187514
GAAGAGC1700.041.0294156
AGAGCAC1700.041.0294158
AGCACAC1700.041.02941510
GTCTGAA1500.040.517
TCTGAAC1500.040.518
ATCTCGT1400.040.1785740
GATCTCG1400.040.1785739
TCTCGTA1350.040.00000441
GTATGCC1550.039.19354645
Produced by FastQC (version 0.11.2)