FastQCFastQC Report
Fri 14 Oct 2016
HL7T3BGXY_n01_ba44.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7T3BGXY_n01_ba44.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences43243508
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACTGACCAATCTCGTATGCC2214950.5122040515306945TruSeq Adapter, Index 4 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG258600.041.6830543
CGTATGC260200.041.4266944
GATCGGA261000.041.4158441
TCTCGTA251750.041.36226341
CTCGTAT252800.041.3489542
ATCGGAA269900.040.709562
CGTCTGA267700.040.7031916
AATCTCG257450.040.45626439
ACACGTC275100.039.70685613
TCGGAAG277000.039.6840443
ACGTCTG275000.039.62284515
ATCTCGT263100.039.62159340
CGGAAGA283800.038.661614
CACGTCT290300.037.6664914
GCACACG293900.037.2058811
CACACGT296950.036.84608512
GTATGCC294900.036.75037445
ACCAATC318450.032.80593536
ACTGACC334300.032.45381532
GACCAAT335500.032.1246235
Produced by FastQC (version 0.11.2)