FastQCFastQC Report
Fri 14 Oct 2016
HL7T3BGXY_n01_ba43.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7T3BGXY_n01_ba43.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44536002
Sequences flagged as poor quality0
Sequence length51
%GC40

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[WARN]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGATGTATCTCGTATGCC1751120.3931920067724085TruSeq Adapter, Index 2 (100% over 51bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTATG195450.041.65944343
CTCGTAT195600.041.51270342
TCTCGTA196100.041.45470441
CGATGTA201500.041.43861434
ACCGATG201600.041.42815432
CGTATGC197250.041.41601644
CACCGAT202800.041.29391531
CCGATGT205450.040.69603333
GATCGGA205300.040.5500141
CGTCTGA208650.040.41469216
GTCACCG207200.040.3514129
ATCGGAA212950.039.6141662
TATCTCG206750.039.49454539
ACACGTC214350.039.33037613
ATCTCGT207600.039.2894440
ACGTCTG215100.039.24514415
TCACCGA217100.038.65673430
TCGGAAG221750.038.094213
CGGAAGA226650.037.310194
CACGTCT231050.036.57497814
Produced by FastQC (version 0.11.2)