FastQCFastQC Report
Mon 24 Oct 2016
HL7MJBGXY_n01_chl24.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7MJBGXY_n01_chl24.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11852975
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGGTAGCATCTCGTATGC430590.36327588643357467TruSeq Adapter, Index 22 (97% over 37bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG53300.060.5428246
GTATGCC61550.052.8260845
CGTATGC63700.050.82246444
ATGCCGT64900.050.1534547
TGCCGTC67600.048.0987148
TCGTATG69000.047.17212743
GCCGTCT73800.044.15274449
CTCGTAT75250.043.0674142
AAAAGGG86000.037.4816170
TCTCGTA90800.035.69217741
GGTAGCA96150.033.45124434
CATCTCG98300.032.897939
GTAGCAT101800.032.00749235
CCGTCTT105050.031.08598550
ACGGTAG105500.030.98382432
GGCGCGC8950.030.50150738
ATCTCGT109100.029.80167840
AAAAAGG110400.029.51490469
TGAAAAA111750.029.47688962
GTCACGG120950.026.9682329
Produced by FastQC (version 0.11.2)