FastQCFastQC Report
Mon 24 Oct 2016
HL7MJBGXY_n01_chl23.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7MJBGXY_n01_chl23.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11843937
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGAGTGGATCTCGTATGC895680.7562350255662454TruSeq Adapter, Index 7 (97% over 36bp)

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG104000.063.50264746
GTATGCC110500.059.894945
ATGCCGT114300.057.87204447
CGTATGC115150.057.5061344
TGCCGTC117000.056.56716548
TCGTATG121550.054.6222143
GCCGTCT123150.053.71360849
CTCGTAT124750.052.462742
AAAAGGG133800.049.14983770
TCTCGTA141550.046.23611541
CGAGTGG150650.043.8376133
CCGTCTT154400.042.97908450
TGAAAAA156000.042.63431562
AAAAAGG157450.041.9462969
ATCTCGT158950.041.3066640
ACGAGTG163400.040.60975632
AGTGGAT163300.040.5053535
GATCTCG165900.039.5129239
CACGAGT168600.039.46071631
CTGCTTG170750.039.0732657