FastQCFastQC Report
Mon 24 Oct 2016
HL7MJBGXY_n01_chl22.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7MJBGXY_n01_chl22.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11344593
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACCGTACGATCTCGTATGC1267711.1174574530791892TruSeq Adapter, Index 22 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG143250.064.86645546
GTATGCC149400.062.38338545
ATGCCGT151900.061.19592347
CGTATGC153300.060.7050144
TGCCGTC155400.059.9320948
TCGTATG159300.058.4625143
GCCGTCT159700.058.2309849
CTCGTAT162950.056.7019442
AAAAGGG170200.054.20483470
TACGATC179050.051.4272136
TCTCGTA180850.051.0897541
CGTACGA182900.050.80370334
CCGTCTT188700.049.54328550
GTACGAT191400.048.40124535
CCGTACG196150.047.5320633
TGAAAAA198100.047.3716162
AAAAAGG196600.047.01548469
CTGCTTG204850.045.6896557
ATCTCGT202650.045.5941940
ACGATCT206250.044.84907537
Produced by FastQC (version 0.11.2)