FastQCFastQC Report
Mon 24 Oct 2016
HL7MJBGXY_n01_chl21.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7MJBGXY_n01_chl21.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences10882075
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTTTCGATCTCGTATGC748480.6878099994716081TruSeq Adapter, Index 21 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG88350.062.86768346
GTATGCC95450.058.37517545
ATGCCGT97200.057.21563347
CGTATGC97700.056.81587644
TGCCGTC98800.056.21976548
TCGTATG104250.053.3458943
GCCGTCT104600.053.13588349
CTCGTAT109050.050.45218742
AAAAGGG115200.047.7277370
TCTCGTA127600.043.0081241
CCGTCTT132200.042.28170450
AAAAAGG136300.040.41699269
TGAAAAA142550.039.28960862
CTGCTTG149500.037.6020357
ATCTCGT147000.037.30789640
CGTTTCG149200.037.08567433
GATCTCG153150.036.0834739
TTCGATC154300.035.36073736
GAAAAAA161700.034.74465663
TTTCGAT161450.034.33667435
Produced by FastQC (version 0.11.2)