FastQCFastQC Report
Mon 24 Oct 2016
HL7MJBGXY_n01_chl20.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7MJBGXY_n01_chl20.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11152784
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGGCCATCTCGTATGC793670.7116339740821663TruSeq Adapter, Index 20 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG89850.062.55854846
GTATGCC96150.058.53314245
ATGCCGT99900.056.3001647
TGCCGTC101000.055.6879948
CGTATGC101000.055.6877444
GCCGTCT105700.053.3108949
TCGTATG107150.052.4259143
CTCGTAT112200.049.97202342
AAAAGGG118800.046.84227470
TCTCGTA129600.043.18179741
CCGTCTT135300.041.98456650
AAAAAGG141750.039.53059469
TGAAAAA144600.039.38484662
CATCTCG142400.038.98077839
GGCCATC143350.038.64867836
CGTGGCC147700.037.7947633
CTGCTTG152000.037.25899557
ATCTCGT153200.036.6896940
ACGTGGC160200.034.99850532
TTGAAAA165100.034.8546561
Produced by FastQC (version 0.11.2)