FastQCFastQC Report
Mon 24 Oct 2016
HL7MJBGXY_n01_chl19.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7MJBGXY_n01_chl19.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences11243804
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTGAAAATCTCGTATGC1970081.7521472270416667TruSeq Adapter, Index 19 (98% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG215100.065.7836346
GTATGCC221350.063.9586545
ATGCCGT224450.063.1056347
CGTATGC225750.062.78930344
TGCCGTC227700.062.17665548
GCCGTCT231250.061.28352449
TCGTATG232650.060.8819443
CTCGTAT237050.059.47003642
AAAAGGG243200.057.77908770
TCTCGTA253300.055.72467841
CCGTCTT257950.054.9820950
AAAAAGG265150.053.02352569
TGAAAAA268900.052.84036362
AATCTCG270950.052.0820239
ATCTCGT273100.051.7104440
CTGCTTG276450.051.4953657
ACGTGAA281450.050.78405432
CGTGAAA284000.050.38968733
TGCTTGA293400.048.4616358
TTGAAAA294150.048.35190661
Produced by FastQC (version 0.11.2)