FastQCFastQC Report
Mon 24 Oct 2016
HL7MJBGXY_n01_chl18.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7MJBGXY_n01_chl18.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12663293
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTCCGCATCTCGTATGC1149540.9077733572144306TruSeq Adapter, Index 18 (100% over 50bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG132950.064.707346
GTATGCC141450.061.0663445
ATGCCGT144950.059.49547247
CGTATGC146400.058.8818444
TGCCGTC149600.057.59961748
TCGTATG153250.056.20402543
GCCGTCT154800.055.64323449
CTCGTAT155450.055.1154742
AAAAGGG167950.051.07571470
GTCCGCA174450.049.09199534
TCTCGTA175000.048.87848741
CGTCCGC181650.047.3386433
CCGTCTT183900.047.01075450
TCCGCAT185550.046.06071535
CCGCATC185600.045.8593836
CATCTCG188050.045.44836839
CGCATCT187450.045.42580837
AAAAAGG193350.044.45650569
ACGTCCG194500.044.2291232
TGAAAAA196100.044.197762
Produced by FastQC (version 0.11.2)