FastQCFastQC Report
Mon 24 Oct 2016
HL7MJBGXY_n01_chl17.fastq.gz

Summary

[OK]Basic Statistics

MeasureValue
FilenameHL7MJBGXY_n01_chl17.fastq.gz
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14351130
Sequences flagged as poor quality0
Sequence length76
%GC46

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACGTAGAGATCTCGTATGC3003402.092796873835022TruSeq Adapter, Index 3 (97% over 37bp)

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TATGCCG324350.066.7079346
GTATGCC333250.064.91588645
ATGCCGT338850.063.8848147
CGTATGC339450.063.70869444
TGCCGTC342200.063.23048448
GCCGTCT347750.062.22112749
TCGTATG347800.062.1890243
CTCGTAT353300.061.0710242
AAAAGGG353000.060.70757370
TCTCGTA375850.057.38929741
CCGTCTT380150.056.97329350
AAAAAGG386150.055.55055669
TGAAAAA393550.055.0204162
CTGCTTG402800.053.7805457
ATCTCGT401050.053.77469640
GATCTCG408900.052.79332739
GAAAAAA423100.051.318763
TGCTTGA426150.050.92445458
TTGAAAA426300.050.8751161
CGTAGAG427850.050.5521533
Produced by FastQC (version 0.11.2)